Chapter 3: the Region Homologous to the X-chromosome Inactivation Centre Has Been Disrupted in Marsupial and Monotreme Mammals
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چکیده
The search for a marsupial X-inactivation centre (and crucially an XIST orthologue) had been ongoing in the Graves laboratory for more than a decade, chiefly by Matthew Wakefield. However, direct searches by Southern blotting, BAC library screening, PCR, RT-PCR and bioinformatic searches had been uniformly unsuccessful. With my supervisor Jenny Graves, I devised a strategy for searching for flanking markers.
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